Cancer Therapy: Preclinical Identification of Lethal microRNAs Specific for Head and Neck Cancer

نویسندگان

  • Marlon Lindenbergh-van der Plas
  • Sanne R. Martens-de Kemp
  • Michiel de Maaker
  • Wessel N. van Wieringen
  • Bauke Ylstra
  • Reuven Agami
  • Francesco Cerisoli
  • C. Ren e Leemans
  • Boudewijn J.M. Braakhuis
  • Ruud H. Brakenhoff
چکیده

Purpose: The prognosis of head and neck squamous cell carcinomas (HNSCC) remains disappointing and the development of novel anti-cancer agents is urgently awaited. We identified by a functional genetic screen microRNAs that are selectively lethal for head and neck cancer cells but not for normal cells. We further investigated the genes targeted by these microRNAs. Experimental Design: A retroviral expression library of human microRNAs was introduced in HNSCC cell lines and normal oropharyngeal keratinocytes to identify tumor-selective lethal microRNAs. Potential downstream gene targets of these microRNAs were identified by gene expression profiling and validated by

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Identification of lethal microRNAs specific for head and neck cancer.

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تاریخ انتشار 2013